Chromatin loop dynamics during cellular differentiation are associated with changes to both anchor and internal regulatory features

Changes in chromatin features predict changes in chromatin looping. (A) R² multiplied by the sign of association for all possible features correlated individually against changes in all loops in the model (top). Heatmap (bottom) is a legend in which the feature, position, and measure for each bar are reported (features: A = ATAC, K = H3K27ac, J = JUN, C = CTCF, R = RAD21, T = RNA; positions: A = anchor, I = interior; measures: M = max, S = sum). (B) R² values calculated for the anchor only, interior only, or all feature models. (C) Scatterplot showing the predicted loop fold-change versus actual loop fold-change for the testing data set for looping. (Gray = static loops, teal = gained loops, maroon = lost loops, R² calculated for all loops included in the testing data set.)