Efficient and accurate KIR and HLA genotyping with massively parallel sequencing data

KIR alleles in CD8⁺ T cells. (A) KIR allele expression fractions in four cells from a colorectal cancer (CRC) patient. (B) The number of expressed KIR alleles in a cell. For example, seven cells expressed two KIR alleles. (C) The number of cells that express the KIR gene, splitting by the cases of single-allele expression or both-allele expression. Only detected KIR genes are displayed. (D) Comparison of the KIR allele fractions between CD39⁻ CD8⁺ T cells and CD39⁺ CD8⁺ T cells. Each line connects a KIR allele in the CD39⁻ cell and the CD39⁺ T cell from the same patient. The P-values are computed with a Wilcoxon signed-rank test and have been adjusted by the Benjamini–Hochberg procedure.