Illustration of the forward genetic screening. (A,B) ODP parameter (A) and SOC (B) of the 23 randomly chosen positive transgenic lines with sgRNAs targeting two or more homoeologs. (ODP) Oleic desaturation proportion, (SOC) seed oil content. Each dot represents a plant, and the IDs of the transgenic plants used for genotyping are marked alongside. The red indicates an increase, and the blue represents a decrease. In A and B, the Student's t-test was used for the statistical analysis. (C) Editing interrogation of the target genes in the 10 selected plants that show significant changes in the fatty acid profiles. The seeds used for the forward genetics were from the plants of the T0 generation.
