CRISPR-Cas9-based repeat depletion for high-throughput genotyping of complex plant genomes

Performances of CRISPR-Cas9-mediated repeat depletion at different multiplexing level and library types. Sequencing reads mapping to the repetitive or single-copy regions with or without CRISPR-Cas9-mediated repeat depletion in (A,B) multiplex libraries containing eight or 96 samples (plx) or (C,D) standard singleplex WGS libraries generated from one L. culinaris cv. Castelluccio, one L. culinaris cv. Redberry, and one L. orientalis sample. Data are means ± SE (n = 3) normalized for the same sequencing input (50 million fragments). Variation percentages observed following CRISPR-Cas9-mediated repeat depletion are reported above each condition.