Multiplexed long-read plasmid validation and analysis using OnRamp

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Figure 4.
Figure 4.

Restriction-digest barcoding for highly similar or clonal plasmids. (A) Diagram of restriction cut-site method for unique read mapping of clonal plasmids using “rotated” references. (B) Number of reads mapping uniquely to each plasmid in a 15-plasmid clonal test pool. (C) Number of reads mapping uniquely to each reference in a nine-plasmid mixed clonal run.

This Article

  1. Published in Advance May 8, 2023, doi: 10.1101/gr.277369.122 Genome Res. 33: 741-749

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