Multiplexed long-read plasmid validation and analysis using OnRamp

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Figure 1.
Figure 1.

OnRamp protocol and pipeline. Pooled plasmids have nanopore adapters added by transposase or by digestion and ligation (A) and then are sequenced (B). (C) Base-called reads are provided to the OnRamp web app, which generates consensus sequences. (D) Consensus sequences are then aligned to user-provided references to identify variation.

This Article

  1. Published in Advance May 8, 2023, doi: 10.1101/gr.277369.122 Genome Res. 33: 741-749

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