Conservation and emergence of neuronal diversity between 6-dpf, 15-dpf, and adult telencephalon. (A) The contribution of select pallium clusters to the total pallium cells in 6-dpf, 15-dpf, and adult pallium. (B) Gene expression profiles of pvalb7 faceted by age. (C) In situ expression domain of pvalb7 in larval (green, RNA-FISH; pale gray, total-Erk; costain for anatomy) and adult telencephalon (magenta, RNA-FISH; yellow, Sytox nuclear stain). Closer views of the left and right expression domains in the adult are shown on the corresponding sides of the complete brain section. The scale bars represent 50 µm. (D) The contribution of select subpallium clusters to the total subpallium cells in 6-dpf, 15-dpf, and adult pallium. (E) Gene coexpression profiles for tubb5 with bhlhe22 and six3b. Statistical test for plots in A and D: unpaired two-tailed t-test with Benjamini–Hochberg correction (Benjamini and Hochberg 1995) for multiple comparisons: (*) P < 0.05, (**) P < 0.01, (***) P < 0.001, (****) P < 1 × 10−4).
