Accurate transcriptome-wide identification and quantification of alternative polyadenylation from RNA-seq data with APAIQ

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Figure 6.
Figure 6.

Experimental validation of the predicted APA events. (A,B) Genome Browser showing an identified tumor-associated IPA event (highlighted in orange) from genes ERCC1 (A) and FLAD1 (B). (C,D) The gel of 3′ RACE experiment (left bottom) and qRT-PCR results of the two isoforms from genes ERCC1 (C) and FLAD1 (D). (E,F) Sanger sequencing results of the amplified transcripts from ERCC1 (E) and FLAD1 (F) by using 3′ RACE experiment. Genomic sequences downstream from cleavage site identified by Sanger sequencing for two IPA were marked in red.

This Article

  1. Published in Advance April 28, 2023, doi: 10.1101/gr.277177.122 Genome Res. 33: 644-657

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