Figure 1.
Overview of the mutation accumulation (MA) experiment. (A) Ancestors were split into multiple lines, which were propagated via single-spore descent. (B) Lines in the MA experiment were transferred by always picking a colony originating from a single spore from a plate, moving this to a test tube with Vogel's medium to allow sporulation, and then diluting spores and spreading them to a sorbose plate. (C) Micrograph of N. crassa mycelium, showing nuclei in fluorescent green and cell walls in red. (D) Distribution of mutations in the MA lines.
