Erasure and retention of histone modifications in early-stage medaka embryos quantitatively revealed by spike-in ChIP-seq. (A) Track views showing representative patterns of each histone modification and IgG control. Enrichment levels of histone modifications after spike-in normalization or that of IgG before spike-in normalization are shown. (B) Genome-wide changes in enrichment of each modification. The average enrichment levels after spike-in normalization (RPKMspike) around all peaks and randomized peaks at each stage are shown as solid lines and dashed lines, respectively (top). Heatmaps showing enrichment levels (RPKMspike) around all peaks (±1 kb from peak center).
