Quality assessment of 15 de novo assemblies produced by HiFi sequencing. (A) PCA plot showing the representation of the genetic diversity of domestic sheep by the 15 samples used for HiFi sequencing. The African Dorper sheep cluster with the European breeds as this breed is developed by crossing European Dorset Horn with the African Blackhead Persian sheep, resulting in its close genetic relationship with European breeds. (B) Repeat content of the 15 de novo assemblies. (C) The difference in chromosome length between the 15 new assemblies and the reference genome (REF, ARS-UI_Ram_v2.0). (D) Number of filled gaps by the different assemblies. (E) Cumulative length of contigs (Nx). In the x-axis, 50% measures the N50 across the new assemblies. HiFi sequencing depth for each assembly is shown with the dashed line referring to the reference genome. Assemblies with higher Nx values are more contiguous. (F) BUSCO completeness for the 15 new assemblies.
