Gene content of the cy0333 MHC-I AG/G region. Representative genes and pseudogenes are shown on the gray bar. The blue boxes highlight a virtually nucleotide-identical ∼47-kb block of sequence located at two distinct positions within the class I AG/G region; this block contains highly similar Mafa-V*01:02 and Mafa-G_pseudo1 genes along with three additional pseudogenes not shown. The pink and green boxes highlight closely related (98.4%) ∼48.5-kb blocks of sequence located just centromeric to each of the virtually nucleotide-identical Mafa-G/Mafa-V blocks. The pink and green boxes contain closely related Mafa-W genes (Mafa-W*01:11 and Mafa-W*01:12, differing at 20 positions across 2196 nt) and closely related Mafa-AG6 genes (Mafa-AG6*04:05:03:03 and Mafa-AG6*04:08:01:01, differing at 36 positions across 2534 nt), as well as three additional closely related duplicated pseudogenes not shown. The position in kilobases relative to the start of the full genomic MHC region is shown across the top. The bottom portion shows the spans of individual ONT reads located within the class I AG/G region. The three ONT reads shown in blue span both copies of the essentially nucleotide-identical Mafa-G/Mafa-V genes.
