The 30 pg of RNA is sufficient to construct a strand-specific RNA sequencing library. (A) PCR results of libraries constructed from different amounts of RNA are shown. (B) The Pearson's correlations among libraries generated by the indicated amounts of RNA. The scatter plots show all detected genes. (RPKM) Reads per kilobase of exon per million reads mapped. (C) Bar graph showing ratios of detected reads at exons and introns among the libraries generated by the indicated amounts of RNA. (D) Bar graph showing the overall detected genes among libraries generated by the indicated amounts of RNA. (E) The reads distributions spanning the gene regions among libraries generated by the indicated amounts of RNA. (TES) Transcription end site. (F) Comparison of sequencing signals between libraries generated by the indicated amounts of RNA. Sense (strand +) and antisense (strand −) transcripts associated with TSS are shown.
