Tn5 is used for strand-specific RNA sequencing. (A) Illustration showing the strategy for strand-specific RNA sequencing. (B) Fragmented and unfragmented RNA were used to generate strand-specific RNA sequencing libraries. (C) The Pearson's correlations between the libraries generated from fragmented and unfragmented RNA. The scatter plots show all detected genes. (RPKM) Reads per kilobase of exon per million reads mapped. (D) Venn diagram showing the overall detected genes between libraries generated from fragmented and unfragmented RNA. (E) Bar graph showing ratios of detected reads at exons and introns. The number of reads mapped to exons or introns was calculated by HOMER. (F) Comparison of sequencing signals between libraries generated from fragmented and unfragmented RNA. Sense (strand +) and antisense (strand −) transcripts associated with transcription start site (TSS) are shown.
