TABLE-seq-generated strand-specific sequencing library from ssDNA. (A) Illustration of how Tn5 transposon tagmented dsDNA and ssDNA. (B) Illustration showing the design for PCR analysis of oligo transposition in ssDNA. (C) PCR results presenting only tagmented dsDNA can be amplified. (D) Illustration showing the design for TABLE-seq. (E) Gel result presenting TABLE-seq method could amplify the tagmented ssDNA. (F,G) Profiles showing the distributions of reconstructed paired-end sequencing fragments forwardly and reversely mapped to the ssDNA. Two independent replicates from repeat #1 (F) and repeat #2 (G) are shown. The x-axis indicates the 5′-to-3′ direction of the template ssDNA. Forward, reconstructed paired-end sequencing reads mapped to the forward direction of ssDNA. Reverse, reconstructed paired-end sequencing reads mapped to the reverse direction of ssDNA. (H) IGV viewing presenting the forward and reverse reads mapped across the ssDNA. (I) The distribution of fragment length in ssDNA TABLE-seq. Two independent replicates from repeat #1 and repeat #2 are shown.
