SARS-CoV-2 genomic RNA contains m6A modification. (A) Identified m6A peaks on SARS-CoV-2 positive strands from B.1, B.1.1.7, and B.1.351 variants are shown. The m6A peaks in the positive strand of SARS-CoV-2 RNA from publicly available data. (B) m6A peaks on the SARS-CoV-2 negative strand. The presence of m6A peaks in the N gene region is highlighted, and normalized coverage is shown on the panel below. (C,D) m6A-RIP qPCR data showing enrichment of N gene region in both the positive strand and the negative strand of viral RNA. (C) Twenty-four hours postinfection with B.1.1.7 in Vero cells; data are represented as a percentage of input, and IgG was used as the negative control. Statistics: two-tailed paired t-test, n = 3; (*) P < 0.05. (D) m6A-RIP qPCR data showing enrichment of the N gene region in both the positive strand and the negative strand of viral RNA from the infected patient samples (n = 5). Data are represented as a percentage of input. IgG was used as a negative control. Statistics: two-tailed paired t-test; (**) P < 0.01.
