Integrative analysis identifies GATA2/3 and MSX2 as candidate regulators of MER41-enhancers in human TSCs. (A,B) Motif configurations of MER41B consensus (A) and one representative MER41-enhancer adjacent to FBN2. (B) The motifs for several known (STAT1, SRF) and novel (GATA2/3, MSX2, GRHL2) TFs of MER41 are labeled in red color. (C) Expression profiles of the four TFs, including GATA2/3, MSX2, and GRHL2, that have placenta-enriched expression. The color gradient indicates the column Z-score based on normalized TPM values. (D) Venn diagram showing the peak overlapping of GATA2/3 and MSX2. (E) Enrichment of MER41 subfamilies in the peaks for GATA2/3, MSX2, and cofactors. (F) Bar plots demonstrate the percentages of MER41-enhancers that are bound by GATA2/3, MSX2, and cofactors. The overlap of MER41-enhancers with TF peaks were determined by using the window function of BEDTools with setting: −w 0. (G) Heatmaps demonstrate the prevalent binding of GATA2/3, MSX2, and cofactors on MER41-enhancers in human TSCs.
