Superior recall of long-read CAGE data for low mappability regions compared to nanoCAGE. (A) Rediscovery rate by nanoCAGE reads as a function of sequencing depth. From the pairwise comparison of active GTSSs detected by nanoCAGE and LRhex peaks from 3 million reads (Fig. 1B), active GTSSs are classified into three groups: (1) detected by both peaks, (2) uniquely detected by LRhex peaks, and (3) uniquely detected by nanoCAGE peaks. For each group, the rediscovery rate by nanoCAGE reads is the number of rediscovered active GTSSs divided by total number of active GTSSs. Active GTSSs are counted as rediscovered if having CAGE TSSs (CTSSs) with at least two nanoCAGE reads within a ±200-bp window. (B) Browser view of NOMO1 GTSS. (C) Browser view of NOMO2 GTSS.
