Figure 5.
Chromatin assembly defects in the absence of CAF-1 result in a transient and S-phase-specific dysregulation of transcription. (A) DNA content measured by flow cytometry in WT and cac1Δ cells at the end of α-factor arrest (af) and 10, 20, 30, 40, 50, 60, and 150 min after release from the initial α-factor arrest. (B–E) Log2 fold-difference of gene expression, measured in TPM, between cac1Δ cells and WT cells. (B,C) Sense transcription. (D,E) Antisense transcription. In both cases, genes are divided into subtelomeric genes (B,D) and nonsubtelomeric genes (C,E).
