Whole-genome long-read sequencing downsampling and its effect on variant-calling precision and recall

Comparison of precision and recall in duplex ONT variant calling versus standard ONT and HiFi. (A) Ratio of log₁₀(1-Statistic) where Statistic is either precision (solid line) or recall (dotted line) of duplex ONT compared to standard ONT sequencing. Anything above the y = 1 line indicates an increase in performance compared to standard ONT and anything below the y = 1 line indicates a decrease in performance compared to standard ONT. (B) Ratio of log₁₀(1-Statistic) where Statistic is either precision (solid line) or recall (dotted line) of duplex ONT compared to HiFi sequencing. Anything above the y = 1 line indicates an increase in performance compared to standard ONT and anything below the y = 1 line indicates a decrease in performance compared to HiFi.