Properties of recombination intermediates. (A) RPA binding in Dmc1+/+ (as in Fig. 2B, but labeled with inferred recombination intermediates). (B) Model for RPA binding after successful strand invasion. RPA binds the DSB-initiating chromosome (black) and the repair-template chromosome (orange) with distinct localization. The dotted lines indicate newly synthesized DNA. (C) Model for RPA binding to the DSB-initiating chromosome subsequent to some DNA resynthesis. (D) Comparison of recombinase (DMC1/RAD51) binding on the wrong side of the break in animals proficient for strand invasion (Dmc1+/+) relative to those defective for strand invasion (Dmc1−/− and Hop2−/−). The signal is highlighted in gold. Hop2−/− data are from Khil et al. (2012). (E) Comparison of the estimated intensity of RPA binding (number of ChIP-seq reads within a peak corrected for background read frequency) in hotspots in Dmc1−/− (x-axis) and Dmc1+/+ (y-axis) in symmetric, asymmetric, and nonpseudoautosomal X Chromosome hotspots, respectively.
