Roles of SEs in cell state maintenance. (A–D) Profiles of DNA interaction and normalized epigenetic signatures depicting the regulatory network of SEs at ATPase (A), Actin (B), IAP (C), and HSP (D) loci. (E) Flow cytometric assay for cell apoptosis detection in JQ1-treated cells. The DMSO treated cells were used as the control. Cells were distinguished as viable (Annexin V-FITC−/PI−), early apoptotic (Annexin V-FITC+/PI−), late apoptotic (Annexin V-FITC+/PI+), and necrotic (Annexin V-FITC−/PI+). (F) Cell viability of JQ1-treated cells. Three biological replicates per treatment were analyzed (n = 3). DMSO treatment was served as the control. Error bar indicates the SD. Two-tailed t-test: (*) P = 0.01764, (**) P = 0.003305 versus the control. (G) qRT-PCR analysis for the expression of SE-associated genes in JQ1-treated cells. Rpl32 was used as the reference gene for data normalization. Three technical replicates for each of the three biological replicates per treatment were analyzed (n = 3). DMSO treatment was served as the control. Error bar indicates the SD. Two-tailed t-test: (*) P < 0.05, (**) P < 0.01, (***) P < 0.001 versus the control.
