Major cell types in adult SVZ and cell type–specific markers based on single-cell profiling. (A) Workflow of sample preparation, single-nucleus RNA-seq, and the downstream analysis. (B) Neurological severity score (NSS) of the control group and mTBI group. Three mice in each group were included. The t-test was used to determine statistical significance, (**) P ≤ 0.01. (C) Bright-field image of the SVZ; the sampling area is circled by the dotted line. (D) Uniform manifold approximation and projection (UMAP) visualization of 15,265 cells from the SVZ of the control and the mTBI group; 17 cell clusters were detected. (E) Heat map showing differentially expressed genes in each cell type; dot plot showing the gene expression level in the indicated GO terms in each cell type. (F) Violin plots showing the expression level of representative genes that were explicitly expressed in each cell population.
