Profiling of ncRNAs during sex determination and differentiation. (A) Experimental design: The offspring from a normal male (ZZ) and a female (ZW) were cultured at 22°C. Under these conditions, some genetic females (ZW) developed into pseudomales. (*) Samples used for RNA-seq. Blue letters in parentheses indicate the symbols for corresponding gonad samples used throughout this paper. (GF) ZW fish prior to sex determination, (GM) ZZ fish prior to sex determination, (F) ZW female fish after sex determination, (M) ZZ male fish after sex determination, (PM) ZW pseudomale fish. (B) Classification of lncRNAs, circRNAs, and miRNAs. (C) Box plots of lncRNA and mRNA expression levels per sample. The x-axis indicates the sample name, and the y-axis indicates the logarithmic values of FPKM. (D) Three dimensional plots of PCA of 15 gonadal samples for expression levels of mRNAs.
