Human lncRNAs mirror adjacent protein-coding gene pre-mRNA expression. (A–C) NIH Roadmap Epigenomics data for loci surrounding protein-coding genes; FOS, TGFBI, and TGIF1. A schematic of each locus is presented, with GENCODE-annotated protein-coding genes shown in black and lncRNAs in green. NIH Roadmap Epigenomics DNase-seq, H3K4me1, and H3K4me3 histone modification ChIP-seq data from 111 uniformly processed human epigenomes are presented. Lines depict mean −log10(P-value) signal, with dark shaded regions indicating 25%–75% percentiles, and lighter shaded regions the 10%–90% percentiles. (D–F) Line plots of z-score normalized protein-coding gene and lncRNA expression values. LncRNA and pre-mRNA were quantified using the expression of the first 10 kb of intronic regions. Mean expression (dark green) and the range of all expression values (shaded light green) is shown for adjacent lncRNAs. Mature mRNA expression is included for comparison.
