Protein-coding genes and lncRNAs exhibit distinct expression dynamics. (A) Schematic representation of the experimental design. Following stimulation, cells were harvested at evenly spaced 10-min intervals, yielding a total of 41 time points. (B) Heat map of lncRNA expression. Each row represents an individual z-score-normalized lncRNA expression profile. Colored bars indicate six clusters obtained through k-means cluster analysis, labeled with the number of transcripts in each. (C) Heat map of mRNA expression, as in B. (D) Comparison of lncRNA and mRNA cluster centroids. Outer boxes display cluster centroids, capturing the mean expression of all cluster members. Shaded regions represent the 5th–95th percentiles of all cluster member expression profiles. Pearson correlation coefficients, displayed in the center boxes, were calculated between all lncRNA and mRNA centroid expression profiles.
