Cell cycle arrest explains the observed bulk 3D genomic alterations in response to long-term heat shock in K562 cells

Chromatin conformation of G₂/M cells changes slightly during LHS. (A) Contact maps in G₂/M-phase cells before and after HS. Chr 6 is shown at 500-kb resolution as in Figure 1A. (B) Contact frequency decay curves in G₂/M-phase cells. (C) Chromatin compartmentalization in G₂/M-phase cells. Chr 6 is shown as an example as in Figure 1C. Autocorrelation matrices, together with the first eigenvector profiles, are shown. (D) Chromosome-wise compartment scores in G₂/M-phase cells in each condition. (E) TADs detected in same region as that shown in Figure 1E are shown as an example for G₂/M cells, together with the corresponding contact maps and insulation score profiles. Detected TADs are shaded. (F) TAD strengths indicated by TAD scores in G₂/M-phase cells at the three conditions. (G) Numbers and proportions of overlap of chromatin loops in G₂/M-phase cells. (H) APA scores for the LHS-specific loops are shown in NHS and SHS conditions.