Compartment-specific and ELAVL1-coordinated regulation of intronic polyadenylation isoforms by doxorubicin

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Figure 2.
Figure 2.

IPA:LE isoform ratio up-regulation by Doxo is more prevalent in the cytosol than in the nucleus. (A) Identification by 3′-seq on MCF-7 cells of abundant IPA isoforms that are regulated by Doxo relative to matched last-exon isoform (IPA:LE ratio) in both cytosol (Cyto) and nucleus (Nuc) or in the cytosol only. The three most prevalent patterns of regulation are indicated. (B,C) RT-qPCR analysis of IPA:LE isoform ratio regulation by Doxo in the indicated genes in the nucleus and cytosol of MCF-7 cells. Data on individual isoforms are also shown for HOMEZ. (*) P < 0.05; (**) P < 0.01; (***) P < 0.001.

This Article

  1. Published in Advance July 20, 2022, doi: 10.1101/gr.276192.121 Genome Res. 32: 1271-1284

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