IPA:LE isoform ratio up-regulation by Doxo is more prevalent in the translatome than in the transcriptome. (A,B) Identification of IPA isoforms regulated by Doxo relative to matched last-exon isoform (IPA:LE ratio) by 3′-seq on whole MCF-7 cells and polysomes. Only abundant IPA isoforms (equalizing at least 5% of matched LE isoform) are shown. (C) Venn diagram comparing IPA:LE regulation events by Doxo in whole cells and polysomes. (D) Visualization of 3′-seq and total-RNA-seq data for the HOMEZ gene in the UCSC Genome Browser and Integrative Genomics Viewer, respectively. (E,F) RT-qPCR analysis of IPA:LE isoform ratio regulation by Doxo in the indicated genes in whole cells and in polysomes. Data on individual isoforms are also shown for HOMEZ. (*) P < 0.05; (***) P < 0.001.
