Scaling of H3 exchange with the replication rate depends on Rtt109. (A,B) Exchange in RTT109-deleted cells is dominated by the replicated fraction, rather than the replication rate: (A,B) As in Figure 2C and 2B, respectively. For all time points, see Supplemental Figure S3. (C) Exchange at the replication fork is decreased in RTT109-deleted cells. The average fork exchange rate in RTT109-deleted cells is plotted against the corresponding rate in wild-type cells. Error bars indicate the SE (n = 3 for rtt109, n = 4 for WT). Color intensity indicates the replicated fraction at loci with the earliest RT, and dot size indicates time. (D) Replication-independent histone dynamics are invariant to RTT109 deletion. Mean myc level of nucleosomes that have not yet been replicated at the indicated time points in wild-type versus RTT109-deleted cells (n = 4 and 3, respectively). Highlighted are the rapidly exchanging nucleosomes (as determined in G1 arrest) used for the linear fit (pink dotted line and equation). The one-to-one line is shown in blue.
