Transcriptome and proteome profiling of the aging heart in genetically diverse mice. DO mice are descended from eight inbred founder strains. Each DO mouse is genetically unique. We obtained cross-sectional samples of tissues from approximately equal numbers of mice aged to 6, 12, or 18 mo. Bulk heart tissue was collected for RNA-seq and mass spectrometry protein analysis. We performed analyses to detect and characterize age-related changes in transcript and protein abundance. We performed functional enrichment of gene sets with age effects, characterized changes in correlation of subunits within protein complexes, and mapped genetic variation that influences age-related changes in transcripts and proteins (age-QTL).
