MYC overexpression leads to increased chromatin interactions at super-enhancers and MYC binding sites

MYC is enriched at both anchors of gained promoter–enhancer chromatin loops regardless of transcriptional activity. (A) Promoter–enhancer chromatin loops are categorized as having promoter-proximal MYC binding or MYC binding at both loop anchors. (B) Percentage of lost (left), common (middle), and gained (right) promoter–enhancer chromatin loops occupied by MYC at the promoter proximal anchor or at both anchors. (C,D) Percentage of (C) MYC-bound and (D) non-MYC-bound transcripts with Hi-C chromatin loops. Transcripts were stratified into top (green), middle (gray), and bottom (yellow) tertiles based on transcript expression after MYC overexpression.