MYC overexpression increases chromatin contact frequency and chromatin loops at super-enhancers. (A) Genome browser view of High MYC and Low MYC cells at the MYC locus. Tracks show Hi-C chromatin loops, virtual 4C signal of interactions at the MYC promoter (red and blue tracks, respectively), H3K27ac and MYC ChIP-seq signal. The difference between the virtual 4C signals of High MYC and Low MYC cells are shown with and without SIQHiC normalization (green tracks). Super-enhancers (red) and typical enhancers (black) are shown as bars below the H3K27ac ChIP-seq tracks. MYC binding peaks are shown as yellow bars below the MYC ChIP-seq tracks. (B) 4C-seq of chromatin interactions at a randomly selected MYC-bound super-enhancer near the PROC gene on Chromosome 2. Tracks show Hi-C chromatin loops, 4C-seq signal (red and blue tracks), difference between 4C-seq signal of High MYC and Low MYC cells (green), H3K27ac and MYC ChIP-seq signal. Gained Hi-C chromatin loop is highlighted in orange. Blue arrows show additional gained chromatin interactions identified using 4C-seq.
