Y′ element subtelomeric sequences have a small effect on telomere length regulation by TEL1 and RIF1. (A) Southern blot of WT, tel1Δ, rif1Δ, and tel1Δ rif1Δ cells with bulk telomere length hybridized with the Y′ and CEN4 probes (arrow). (B) Individual telomere lengths determined by nanopore for telomere ends containing Y′ subtelomeric elements reported for tel1Δ, rif1Δ, and tel1Δ rif1Δ cells. (C) Southern blot of WT, tel1Δ, rif1Δ, and tel1Δ rif1Δ samples hybridized with unique probe to TEL01L and CEN4 probe (arrow) to examine unique TEL01L with no subtelomeric elements. (D) Individual telomere lengths determined by nanopore for telomere ends containing only X subtelomeric elements or no telomeric elements (TEL01L) reported for tel1Δ, rif1Δ, and tel1Δ rif1Δ cells. A multiple t-test was used to assess the significance of telomere length differences between genotypes, and P-values are reported as (*) <0.05, (**) <0.01, (***) <0.001, (****) <0.0001, or (n.s.) not significant.
