Telomere fragment length measured by nanopore sequencing accurately reflects length measurements by Southern blot. (A) Southern blot probed with Y′ subtelomeric probe and with CEN4 probe (arrow) as a marker at 1.4 kb to calibrate for densitometry. Two replicates of wild-type (WT) cells and two replicates of rif1Δ cells are shown for each strain, UE1 and UE11. (B) Comparison of densitometry (dotted lines) of Southern blot shown in A and nanopore sequencing (histogram fill bars) for UE1 strain. The light orange bars represent nanopore read counts for WT cells; the light purple bars represent nanopore read counts for rif1Δ cells. The spike in densitometry data at 1400 bp is owing to the CEN4 internal control signal. (C) Southern blot probed with the unique single telomere TEL01L probe and CEN4 probe (arrow). (D) Comparison of densitometry (dotted lines) of Southern blot in C and nanopore sequencing (bars) of UE1 TEL01L. The light orange bars represent nanopore read counts for WT cells; the light purple bars represent nanopore read counts for rif1Δ cells.
