Experimental validation of dopaminergic regulatory signature. (A) Representative micrographs showing GFP expression driven by six different genomic regions overlapping predicted dopaminergic regulatory signature windows. Dopaminergic expression was assessed by colocalization with otIs181 reporter (dat-1p::mcherry; ttx-3p::mcherry) shown in red. Except for F59F3.6, which is exclusively expressed in dopaminergic neurons, GFP expression was also found in additional unidentified and reporter-specific neurons in the head. See Supplemental Table S8 for quantification of two independent reporter lines for each construct. Scale: 25 µm. (B) Distance from the central location of the construct to ATG. Constructs driving GFP expression in dopaminergic neurons tend to locate at closer distances to the ATG than those not active in dopaminergic neurons. Two-tailed t-test.
