Fast and accurate mapping of long reads to complete genome assemblies with VerityMap

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Figure 4.
Figure 4.

VerityMap detects a deletion in CHM13-Cen10-interim that is absent from the publicly released CHM13 assembly. The top panel shows the distance concordance test applied to CHM13-Cen10-interim (left) and Cen10 in CHM13 assembly (right). The bottom panel shows read alignments ∼6 Mbp in two versions of the assembly. (Bottom left) VerityMap reveals a deletion of length ∼2.4 kbp in CHM13-Cen10-interim. The remaining peaks correspond to the Het sites with various multiplicities in the assembly. In particular, one heterozygous deletion of ∼1.7 kbp that is supported by ∼60% of reads is only 20 kbp upstream of the misassembly site. (Bottom right) The misassembly is corrected in the current version of Cen10 assembly, whereas the putative Het sites remain.

This Article

  1. Genome Res. 32: 2107-2118

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