daf-2 mutations contribute to changes in mRNA levels and biological processes that match chronological and physiological aging. (A) Multiple dimension scaling analysis of mRNA levels in wild-type (WT) and daf-2(e1370) [daf-2(-)] animals at indicated ages. (B) The number of mRNAs with levels that matched physiological aging that is categorized to temporal shift and slope change, and chronological aging. Red and blue bars represent up-regulated and down-regulated terms, respectively, during aging. (C) Overrepresented WormCat terms of genes identified in B. P values were calculated by using a hypergeometric test. (D) The number of Gene Ontology biological process (GO-BP) terms that matched physiological aging that are divided into temporal shift and slope change, and chronological aging. Red and blue bars represent up-regulated and down-regulated terms during aging, respectively. (E) Normalized enrichment of age-dependent down-regulated terms associated with slope change. Terms were sorted based on the maximum absolute normalized enrichment in descending order.
