Figure 2.
Schematic of MPRA. (A) Sequence alignment of motif-focused regions to test primate and ancestral reconstructed LTR18A elements. MAFK and DBP motif regions are boxed. (B) Tiling of ancestral and hg19 genomic LTR18A elements in a reconstructed phylogenetic tree. All elements were tiled with 160-bp tiles at 10-bp intervals. (C) Plasmid construct and enrichment score calculation. Each LTR18A fragment was integrated upstream of a minimal promoter (minP) and tagged with 10 unique barcodes (BCs) during library synthesis. The MPRA library was transfected into HepG2 and K562 cells. Enrichment scores are log2 ratios of RNA/DNA normalized to basal.
