LTR18A ancestral reconstruction. (A) Model of LTR18A evolution split into transposition and speciation phases. Computational reconstruction was performed for ortholog ancestors and transposition intermediates using PRANK. (B) Phylogenetic tree for reconstructed transposition intermediates and ortholog ancestors at leaves. Ancestral node 43 (Anc43) is labeled in red, as well as the edges to ortholog ancestors that contain the 27-bp insert. The subfamily ancestor at ancestral node 45 (Anc45) is labeled by the purple dot. (C) Alignment of Repbase consensus (top), Anc45 (#45#, middle), and Anc43 (#43#, bottom). Motifs in the sequences are boxed. DBP is shown to represent CEBP-related motifs.
