Figure 1.
Measurements of a core promoter library at four genomic locations by patchMPRA. (A) Schematic of gene regulation by the core promoter, adjacent cis-regulatory sequences, and the genomic environment. (B) Schematic of patchMPRA method (see Methods for details). tagBFP: blue fluorescent protein; HSV-TK: herpes simplex virus thymidine kinase; gBC: genomic barcode; pBC: promoter barcode. (C) Reproducibility of core promoter measurements from independent patchMPRA transfections. (D) The expression of all core promoters in the library at each genomic location.
