Persistent Mediator association preferentially occurs at Hmo1-binding RP genes repressed by heat shock. (A) Heat maps and line graphs showing normalized occupancy of Med15 and Med18 in kin28AA yeast treated with rapamycin, with and without heat shock, at RP genes divided into Hmo1-binding, non-Hmo1-binding, and Abf1-binding genes (Supplemental Table S2). (B) Browser scans showing occupancy of Med15 and Med18 in kin28AA yeast treated with rapamycin, with and without heat shock, and Rap1 and Hmo1 in non-heat-shocked yeast, at RPS24A and RPL21A (Hmo1-binding) and RPL40B and RPS9B (non-Hmo1-binding). Scale, in reads per million mapped reads, is indicated for each scan. (C) Heat maps and line graphs showing normalized occupancy of Med15 and Med18 at UAS genes, Hsf1 targets, and Rap1-binding RP genes that do or do not bind Hmo1, in wild-type (BY4741) and hmo1Δ yeast after 15 min of heat shock.
