Sequence-based correction of barcode bias in massively parallel reporter assays

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Figure 2.
Figure 2.

MTSA accurately predicts tag sequence effects on expression. (A) Observed relative expression is compared to its predicted value from tag sequence with fivefold cross-validation using MPRA data from Ulirsch et al. (2016). (B,C) MTSA correction improves the correlation between two randomly selected tags from each CRE: log2 transformed raw expression (B) and MTSA-corrected expression (C) are compared.

This Article

  1. Genome Res. 31: 1638-1645

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