Figure 1.
Propagation of MA lines. The common ancestor of the MA lines (G0) was thawed from a cryopreserved sample and a single immature hermaphrodite picked onto an agar plate. Lines were propagated by single-worm transfer at 4-d (one generation) intervals for t = Gmax transfers. Lines were initially genetically homogeneous (pink chromosome pairs); colored bars represent new mutations, which are fixed in a line with expectation u = (1 – s)/(2 – s), where s is the selection coefficient (Keightley and Caballero 1997). For details of the MA experiments, see Methods and Supplemental Table S1.
