
Iso-Seq full-splice matches have different transcription start sites (TSSs) and transcription termination sites (TTSs) compared with that of Ensembl annotations. Full-splice matches are isoforms that have the same exon boundaries as Ensembl transcripts (6610 isoforms). (A) On average, full-splice match isoform TSSs are located 99 bp upstream of the annotated TSS. (B) Full-splice match isoform TTSs are located on average 500 bp upstream of the annotated TTS.











