Hydroxycarbamide effects on DNA methylation and gene expression in myeloproliferative neoplasms

Comparison of homologous differentially expressed genes between human and mouse. Venn diagrams show the total number of DEGs commonly regulated in human and mouse in neutrophils (A) and stem cells (CD34⁺ and LSK cells in human and mouse, respectively) (B). (C) Venn diagram shows the total number of genes identified in Clusters 2 and 4 from mouse LSK cells that overlap the DEGs from human CD34⁺ cells. (A,C) Heatmap plots represent the log₂(fold change) of treated samples per patient versus pretreatment samples, along with pairwise comparison of each mouse condition (Jak2VF VEH vs. WT-VEH, WT-HC vs. WT-VEH, and Jak2VF-HC vs. Jak2VF-VEH). Bold is used to indicate the genes with similar expression between human and WT-HC or Jak2VF-HC mice.