Enzymatic methyl sequencing detects DNA methylation at single-base resolution from picograms of DNA

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Figure 1.
Figure 1.

Enzymes involved in the detection of 5mC and 5hmC. (A) TET2 catalyzes the oxidization of 5mC to 5hmC, 5fC, and 5caC in three consecutive steps. (B) The T4-phage enzyme T4-BGT glucosylates pre-existing genomic 5hmC as well as 5hmC formed by the action of TET2 to 5-(β-glucosyloxymethyl)cytosine (5gmC). (C) APOBEC3A deaminates cytosine, 5mC, and, to a lesser extent, 5hmC.

This Article

  1. Published in Advance June 17, 2021, doi: 10.1101/gr.266551.120 Genome Res. 31: 1280-1289

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