Hi-C contact maps in zebrafish sperm display “hinge-like” domains. (A) Correlation matrix from Chr 13 (top) and Chr 24 (bottom) for zebrafish sperm (left) and 24 hpf (right). First principal component values to determine A/B compartment status (below). (B) Contact matrices from two regions Chr 13: 33.4–39.4 Mb (top) and Chr 24: 15.6–21.6 Mb (bottom) for sperm (left) and 24 hpf (right), 25-kb resolution in log scale. Flares detected in sperm are marked by black arrows and are not evident in 24 hpf embryos. (C) Contact maps for 24 hpf and sperm samples each presented for a 6-Mb region on Chr 24 at 25-kb resolution in log scale (top). First principal component values to determine A/B compartment status (middle). Heatmap of insulation scores for different window sizes (bottom). The hinge region is marked by a purple square, and distance is marked by an orange square.
