Assignment of FCR monomers to chromosomes by k-mer content. (A) Clustered heat map showing the presence (blue) or absence (white) of individual enriched k-mers on each centromeric genome segment. Both rows and columns are clustered to show k-mers and segments that display similar distributions. Genome segments, on the y-axis, are labeled on the left side indicating the L subgenome (blue), S subgenome (red). (B) Similar to A, but the genome segments, y-axis, are not ordered based on similar k-mer content and are instead listed by chromosome. L subgenome chromosomes are shaded with gray for clarity. (C) Clustered heat map of chromosomes by abundance of Cenpa-enriched k-mers. By combining 50-kb genome segments from each chromosome, an array of counts for each k-mer was used to generate a Euclidean distance between chromosomes used for clustering. Coloring of heat map is (1 − Euclidean distance). (D) Clustered heat map of counts reported from Bowtie of the number of times any k-mer from each FCR monomer aligns to each chromosomal contig.
