RSC is generally required for repair of CPD lesions in yeast, whereas SWI/SNF is not. (A,B) UV sensitivity of yeast mutants in the RSC (rsc1Δ and rsc2Δ) or SWI/SNF complex (snf6Δ). (C,D) UV sensitivity of rad26Δrsc2Δ and rad16Δrsc2Δ double mutants compared with single mutants. (E) Representative alkaline gel of CPD repair in WT and snf6Δ-mutant cells. Genomic DNA was isolated at the indicated time following damage induction with 100 J/m2 UVC light and treated with or without (+/−) T4 endonuclease V. (F–I) Quantification of CPD repair in RSC or SWI/SNF mutants based on alkaline gel analysis. The percentage of CPDs repaired is plotted as the mean ± SEM of a minimum of three replicates. P-values were calculated using an unpaired t-test with Holm–Sidak correction for multiple hypothesis testing. (*) P ≤ 0.05.
